Preparative Regimen
Patients will receive hydroxyurea and azathioprine starting day -59 to day -11, fludarabine on Days -15, -14, -13, -12, -11; busulfan twice daily on Days -10, -9, -8, -7; defibrotide beginning on day -10 and continuing through Day +21; thiotepa on day -6; cyclophosphamide on Days -5, -4; TLI on day -2; rabbit ATG on day -5,-4,-3, and -2. GVHD prophylaxis will consist of tacrolimus for 100 days post SCT and then tapered if ≤grade I AGVHD.
Patients will receive hydroxyurea and azathioprine starting day -59 to day -11, fludarabine on Days -15, -14, -13, -12, -11; busulfan twice daily on Days -10, -9, -8, -7; defibrotide beginning on day -10 and continuing through Day +21; thiotepa on day -6; cyclophosphamide on Days -5, -4; TLI on day -2; rabbit ATG on day -5,-4,-3, and -2. GVHD prophylaxis will consist of tacrolimus for 100 days post SCT and then tapered if ≤grade I AGVHD.
Reference: Freed J, Talano J, Small T, Ricci A, Cairo MS. Allogeneic cellular and autologous stem cell therapy for sickle cell disease: 'whom, when and how'. Bone Marrow Transplant. 2012;47(12):1489-1498.